Journal: Data in Brief

Article Title: Data supporting characterization of CLIC1, CLIC4, CLIC5 and DmCLIC antibodies and localization of CLICs in endoplasmic reticulum of cardiomyocytes

doi: 10.1016/j.dib.2016.03.061

Figure Lengend Snippet: Sequence of the primers used in real time PCR.

Article Snippet: After loading the cells were fixed, permeabilized, and labeled with anti-CLIC1 [0.2 μg/mL, SC-271051 (lot no: E1711), Santa Cruz], CLIC4 [0.2 μg/mL, SC-135739 (lot no: D1911), Santa Cruz] and CLIC5 [0.2 μg/mL, ACL-025 (lot no: ANO102) Alomone lab] antibodies as mentioned above. (2) Adult rat cardiomyocytes: Dissociated cardiomyocytes were immediately transferred onto poly- l -lysine coated coverslips for 1 h at 4 °C and then loaded with 200 nM mitotracker for 10 min at 37 °C.

Techniques: Sequencing

Journal: Data in Brief

Article Title: Data supporting characterization of CLIC1, CLIC4, CLIC5 and DmCLIC antibodies and localization of CLICs in endoplasmic reticulum of cardiomyocytes

doi: 10.1016/j.dib.2016.03.061

Figure Lengend Snippet: Specificity of CLIC antibodies using CLIC1, CLIC4 and CLIC5 knock out (KO) mice heart lysates . (I) 50 µg of heart lysates from WT, clic1 −/− , clic4 −/− and clic5 −/− mouse were electrophoresed on 4–20% (w/v) SDS-PAGE, transferred onto nitrocellulose membrane and probed with anti-CLIC1, anti-CLIC4 and anti-CLIC5 antibodies. Absence of CLIC1, CLIC4 and CLIC5 specific bands in KO organ samples confirms the specificity of the antibody. Corresponding Ponceau S stained nitrocellulose membrane is shown as a loading control.

Article Snippet: After loading the cells were fixed, permeabilized, and labeled with anti-CLIC1 [0.2 μg/mL, SC-271051 (lot no: E1711), Santa Cruz], CLIC4 [0.2 μg/mL, SC-135739 (lot no: D1911), Santa Cruz] and CLIC5 [0.2 μg/mL, ACL-025 (lot no: ANO102) Alomone lab] antibodies as mentioned above. (2) Adult rat cardiomyocytes: Dissociated cardiomyocytes were immediately transferred onto poly- l -lysine coated coverslips for 1 h at 4 °C and then loaded with 200 nM mitotracker for 10 min at 37 °C.

Techniques: Knock-Out, SDS Page, Membrane, Staining, Control

Journal: Data in Brief

Article Title: Data supporting characterization of CLIC1, CLIC4, CLIC5 and DmCLIC antibodies and localization of CLICs in endoplasmic reticulum of cardiomyocytes

doi: 10.1016/j.dib.2016.03.061

Figure Lengend Snippet: Localization of CLIC1, CLIC4 and CLIC5 to the endoplasmic reticulum. (I) Isolated p3 neonatal cardiomyocytes were loaded with ER tracker (A, E, I), fixed, permeabilized, labeled with anti-CLIC1 (B), anti-CLIC4 (F), anti-CLIC5 (J) antibodies and further stained with DAPI (C, G, K). D, H and L are merge images showing colocalization of CLIC1 and CLIC4 to the endoplasmic reticulum whereas CLIC5 shows much less colocalization. D′, H′ and L′ are enlarged images of the squared region. (II) Bar graph representing percentage colocalization of CLIC1, CLIC4 and CLIC5 to endoplasmic reticulum ( n =3).

Article Snippet: After loading the cells were fixed, permeabilized, and labeled with anti-CLIC1 [0.2 μg/mL, SC-271051 (lot no: E1711), Santa Cruz], CLIC4 [0.2 μg/mL, SC-135739 (lot no: D1911), Santa Cruz] and CLIC5 [0.2 μg/mL, ACL-025 (lot no: ANO102) Alomone lab] antibodies as mentioned above. (2) Adult rat cardiomyocytes: Dissociated cardiomyocytes were immediately transferred onto poly- l -lysine coated coverslips for 1 h at 4 °C and then loaded with 200 nM mitotracker for 10 min at 37 °C.

Techniques: Isolation, Labeling, Staining

Journal: Acta Pharmacologica Sinica

Article Title: Pharmacokinetic and pharmacodynamic evidence for developing an oral formulation of octreotide against gastric mucosal injury

doi: 10.1038/aps.2017.159

Figure Lengend Snippet: The expression levels of the main SSTRs in rat tissues, and the influence of SSTRs on the distribution of OCT in rats. (A) The relative expression levels of SSTR2, SSTR3 and SSTR5 in rat stomach, duodenum, jejunum, ileum and colon; (B) The expression of SSTR2 in rat stomach sections; (C) The expression of SSTR3 in rat stomach sections as measured by fluorescence immunoassay; (D) The expression of SSTR5 in rat stomach sections; (E) The effects of SSTR2 antagonists on the distribution of OCT. * P<0.05.

Article Snippet: After washing with phosphate-buffered solution (PBS, pH 7.4) three times, the stomach slices were blocked with 10% fetal calf serum (FCS) for 30 min. Polyclonal rabbit anti-mouse SSTR2, SSTR3 and SSTR5 primary antibodies (Alomone Lab, Jerusalem, Israel) were used at dilutions of 1:500, 1:250 and 1:250, respectively.

Techniques: Expressing, Fluorescence